BiosampleCellCulture from May 5th, 2017

released

Homo sapiens A549 immortalized cell line

   May 5th, 2017 at 6:42pm

Details


aliases 
  1. dekker-lab:BiosampleCellCulture_A549_Homo-sapiens-A549-ENCBS009OIC
culture_duration 
14
culture_harvest_date 
2010-03-25
culture_start_date 
2010-03-11
dbxrefs 
  1. ENCODE:ENCBS009OIC
in_vitro_differentiated 
No
karyotype 
This is a hypotriploid human cell line with the modal chromosome number of 66, occurring in 24% of cells. Cells with 64 (22%), 65, and 67 chromosome counts also occurred at relatively high frequencies; the rate with higher ploidies was low at 0.4%. There were 6 markers present in single copies in all cells. They include der(6)t(1;6) (q11;q27); ?del(6) (p23); del(11) (q21), del(2) (q11), M4 and M5. Most cells had two X and two Y chromosomes. However, one or both Y chromosomes were lost in 40% of 50 cells analyzed. Chromosomes N2 and N6 had single copies per cell; and N12 and N17 usually had 4 copies. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.
passage_number 
5
public_release 
July 26th, 2017