BiosampleCellCulture from October 16th, 2018

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HeLa cells were cultured in DMEM containing 10% fetal bovine serum and 1% penicillin/streptomycin at 37C in 5% CO2. Cells were transfected with SF3B1 siRNA and cultured for 72 hours, and croslinked.

   October 16th, 2018 at 10:34pm

Details

aliases 
  1. waldman-lab:insituDNaseHiC-HeLa_SF3B1_culture_rep2
cell_line_lot_number 
HeLa (ATCC-CCL-2)
contributing_labs 
  1. William Noble, UW
culture_duration 
4
culture_harvest_date 
2018-01-29
culture_start_date 
2018-01-25
doubling_number 
Four
doubling_time 
24.67 hours
karyotype 
70-90 chromosomes
morphology_image 
Image from 2018-10-16
passage_number 
3
protocols_additional 
  1. 4DN Standard DNase Hi-C protocol
public_release 
November 20th, 2018