released
Patski cells are cultured in DMEM (Dulbecco's Modified Eagle Medium) with 10% FBS and 1% penicillin/streptomycin. To induce DNA demethylation a 48h treatment using 4µM 5-aza-2’-deoxycytidine (5-aza-2dC) was used, followed by a 24h recovery period.
February 6th, 2018 at 7:49pm
Details
Attribution
Details
aliases
- disteche_lab:pastki_wt_5aza_rnaseq_culture_rep2
cell_line_lot_number
Patski 1998 stock
contributing_labs
culture_duration
3
culture_harvest_date
2016-06-29
culture_start_date
2016-06-26
doubling_time
1 day
karyotype
Most cells have a near dipoid karyotype
passage_number
3
public_release
February 16th, 2018