Biosample

4DNBS8Q2TT5U
released
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CRISPR/cas9-modified Patski cells (Del-hinge clone a) with 4uM 5-aza treatment prepared for RNA-seq; biological replicate 1

   February 6th, 2018 at 9:51pm

Overview

Stable Genomic Modifications 
Crispr deletion of 127 kb 'hinge' region near Dxz4 gene
Treatment 
Biosample Protocols 
Biosample preparation protocol from 2017-12-15

Biosource

Biosource 
Patski - 4DNSRJ2LKIV4
Biosource Type 
Immortalized cell line
Cell Line Name 
Patski
Individual 
4DNINWJ51C9L
Biosource Vendor 
Disteche Lab

Cell Culture

Cell Culture Information 
BiosampleCellCulture from 2018-02-06
Description 
Patski cells are cultured in DMEM (Dulbecco's Modified Eagle Medium) with 10% FBS and 1% penicillin/streptomycin. To induce DNA demethylation a 48h treatment using 4µM 5-aza-2’-deoxycytidine (5-aza-2dC) was used, followed by a 24h recovery period.
Synchronization Stage 
None
Morphology Image 
None
Total Days in Culture 
3
Culture Start Date 
June 26th, 2016 12:00am