Biosample

4DNBSGDOOBM2
released
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CRISPR edited human induced pluripotent stem cells - 80 kb del

   January 22nd, 2021 at 12:19am

Overview

Stable Genomic Modifications 
Crispr deletion for 80KB IDS deletion
Treatment 
None
Biosample Protocols 
None

Biosource

Biosource 
7889SA3.5 - 4DNSRIOTVJ4X
Biosource Type 
Induced pluripotent stem cell
Cell Line Name 
pluripotent stem cell
Individual 
4DNINF2U62Q5
Biosource Vendor 
Tessier-Lavigne Lab

Cell Culture

Cell Culture Information 
BiosampleCellCulture from 2021-01-19
Description 
Culture conditions for iPS cells with a Crispr deletion of 80 kb at an early/late replication IZ boundary prepared for 5C - Cells were grown on matrigel in mTESR+ media and passaged when reaching ~ 70% confluency using versene. NOTE: Cells were isolated as single cell clones after CRISPR modification and grown up to establish cultures
Synchronization Stage 
None
Morphology Image 
None
Total Days in Culture 
34
Culture Start Date 
October 27th, 2019 12:00am