Included Properties
Data Type
Dataset
Sample Category
Sample
Assay Details
Set Type
Warnings
15 Common Properties
Project
4DN
Lab
Bing Ren, UCSD
Experiment Category
Microscopy
Experiment Type
FISH
Organism
Mouse
Sample Type
stem cells
Sample Tier
Unclassified
Organ/System
No value
Tissue Source
stem cell
Publication
Huang H et al. (2021) PMID:34002095
Modifications
RMCE
Treatments
No value
Center
NOFIC - Ren
Status
Released
Commendations
No value
Title
Experiment Type
Biosample
Dataset
Condition
Assay Details
Lab
multiplexed FISH
F123-CASTx129 with Sox2 tags and RMCE site downstream of Sox2 SE
Chromatin Tracing of Sox2 and SE with engineered CTCF insertions
4 CTCF sites inserted downstream of both Sox2 and SE with Sox2 RNA FISH
Target
42 consecutive 5-kb loci on chr3 (mouse), Chromosomes, Sox2 mouse transcript, GFP transcript, mCherry transcript
multiplexed FISH
F123-CASTx129 with Sox2 tags and RMCE site between Sox2 and its SE
Chromatin Tracing of Sox2 and SE with engineered CTCF insertions
4 CTCF sites inserted between Sox2 and SE with Sox2 RNA FISH
Target
42 consecutive 5-kb loci on chr3 (mouse), Chromosomes, Sox2 mouse transcript, GFP transcript, mCherry transcript
multiplexed FISH
F123-CASTx129 with Sox2 tags and RMCE site between Sox2 and its SE
Chromatin Tracing of Sox2 and SE with engineered CTCF insertions
4 mutant CTCF sites inserted between Sox2 and SE
Target
42 consecutive 5-kb loci on chr3 (mouse), Chromosomes
multiplexed FISH
F123-CASTx129 with Sox2 tags and RMCE site downstream of Sox2 SE
Chromatin Tracing of Sox2 and SE with engineered CTCF insertions
4 CTCF sites inserted downstream of both Sox2 and SE
Target
42 consecutive 5-kb loci on chr3 (mouse), Chromosomes
multiplexed FISH
F123-CASTx129 with Sox2 tags and RMCE site between Sox2 and its SE
Chromatin Tracing of Sox2 and SE with engineered CTCF insertions
4 CTCF sites inserted between Sox2 and SE
Target
42 consecutive 5-kb loci on chr3 (mouse), Chromosomes
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