released
The backbone of this construct is pUC19, we requested from addgene 103854. For TERRA guide sequence, we synthesize the oligos and replace the original target sequence via bbsI restriction enzyme.
November 4th, 2021 at 3:10pm
Details
Attribution
Details
aliases
- huaiying-zhang-lab:terra_grna_construct
construct_type
crispr construct
name
TERRA-gRNA
public_release
November 16th, 2021