ExperimentSetReplicate

ExperimentSet4DNES1U49MZQ
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Single particle tracking experiments (n = 29) on Drosophila embryos carrying the construct parS-homie-noPr-PP7 (control without promoter), located at 142kb upstream to the eveMS2 allele (2R:9,836,451, dm6). Male flies with this construct were crossed with MCP::3xtagBFP/PCP::3xmKate2; parB::eGFP/+ females to produce embryos for imaging.

   January 13th, 2021 at 11:42pm
Source Publication
Dynamic interplay between enhancer-promoter topology and gene activity.
Chen H, Levo M, et al., Nature genetics 2018

Note on replicates  

This Experiment Set collects many replicates (the number is indicated in the description above). The complete processed data from all of the replicates in this set can be found in the Processed File. A Raw File (from one replicate) is presented as an example, for exploratory purposes.

Assay Description  

Experiment Set Properties  

Set Type 
Replicate
Organism 
D. melanogaster
Sample Type 
whole organisms
Sample 
Drosophila embryo
Experiment Type(s) 
SPT
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: MS2-tagged endogenous eve transcript and enhancer (fruit fly), ParS-tagged homie (no promoter) cassette (engineered reagent), PP7-tagged transcript from homie (no promoter) cassette (engineered reagent)
Sample Image 
Sample image snapshot from live cell imaging of a Drosophila embryo with parS-homie-noPr-PP7 eveMS2
View File Item - 4DNFIUTWRT8J
Channel
Path
 
ch00
MS2-tagged endogenous eve transcript and enhancer (fruit fly) targeted by mTagBFP2-labeled MCP
ch01
PP7-tagged transcript from homie (no promoter) cassette (engineered reagent) targeted by mKate2-labeled PCP
ch02
ParS-tagged homie (no promoter) cassette (engineered reagent) targeted by eGFP-labeled ParB

1 Processed File

Experiment
File
File Type
File Size
From Multiple Experiments
4DNES1U49MZQ
Multiple Experiments
File
4DNFILJ2WX5N
spt results, submitter format (csv)
39.78 MB