ExperimentSetReplicate

ExperimentSet4DNES3HRUEMD
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Immunofluorescence on HeLaNCAPH cells imaged for Rad21 300m after release from arrest

   September 13th, 2019 at 10:07pm
Source Publication
A chromosome folding intermediate at the condensin-to-cohesin transition during telophase.
Abramo K, Valton AL, et al., Nature cell biology 2019

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
immortalized cells
Sample 
HeLa cell line with NCAPH insertion
Experiment Type(s) 
Immunofluorescence
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: Chromosomes, RAD21, NCAPH protein, TUBA4A protein
Sample Image 
Immunofluorescence sample image
View File Item - 4DNFI4LJLWZT
Channel
Path
 
ch00
Chromosomes targeted by DAPI
ch01
RAD21 targeted by Alexa Fluor 488-labeled Rad21 Antibody (ab154769)
ch02
NCAPH protein targeted by dTomato-labeled NCAPH
ch03
TUBA4A protein targeted by Alexa Fluor 647-labeled Tubulin Antibody (SigmaT6199)

1 Processed File

Experiment
File
File Type
File Size
Experiment
4DNEX8ZWPLGZ
Bio Rep 1, Tec Rep 1
Immunofluorescence on HeLa cell line with NCAPH insertion
File
4DNFIYOKVCZJ
counts (csv)
703 Bytes