ExperimentSetReplicate

ExperimentSet4DNES51KYEOA
released
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FISH/IF of Chromosome 19-Control and Chromosome 18-Control2 and Nucleolus and Nuclear Speckle

   May 5th, 2018 at 4:05am
Source Publication
Higher-Order Inter-chromosomal Hubs Shape 3D Genome Organization in the Nucleus.
Quinodoz SA, Ollikainen N, et al., Cell 2018

Assay Description  

Experiment Set Properties  

Set Type 
Replicate
Organism 
M. musculus
Sample Type 
stem cells
Sample 
F1 2-1 mESC line
Experiment Type(s) 
DNA FISH
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: GRCm38:19:44925701-46925611 mouse region, GRCm38:18:75840470-76761410 mouse region, Ncl mouse protein, Srsf2 mouse protein
Sample Image 
4DNFIFLMIBTD.jpeg
View File Item - 4DNFIFLMIBTD
Channel
Path
 
ch00
GRCm38:19:44925701-46925611 mouse region targeted by 429/470nm fluorophore-labeled oligo probe set
ch01
GRCm38:18:75840470-76761410 mouse region targeted by 495/517nm fluorophore-labeled oligo probe set
ch02
Ncl mouse protein targeted by rabbit anti-nucleolin antibody (with Alexa Fluor 568-labeled anti-rabbit secondary antibody)
ch03
Srsf2 mouse protein targeted by mouse anti-SC35 antibody (with DyLight650-labeled anti-mouse secondary antibody)

4 Processed Files

Experiment
File
File Type
File Size
Experiment
4DNEX1GI9T7B
Bio Rep 1, Tec Rep 1
DNA FISH on F1 2-1 mESC line
File
4DNFIIN5PVGW
locus distances, submitter format (csv)
2.44 kB
File
4DNFIQIEICOL
locus distances, submitter format (csv)
653 Bytes
File
4DNFIXPDSNT8
locus distances, submitter format (csv)
2.51 kB
File
4DNFI2M63KHZ
locus distances, submitter format (csv)
395 Bytes