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Cohesin is a ring complex composed of four core  subunits and seven regulatory subunits. In an effort to comprehensively identify  additional cohesin-interacting proteins, we used gene editing to introduce a dual epitope tag into the endogenous allele of each of 11 known components of cohesin  in cultured human cells, and we performed MS analyses on dual-affinity purifications. In addition to reciprocally identifying all known components of cohesin, we found that cohesin interacts with a panoply of splicing factors and RNA-binding proteins (RBPs). These included diverse components of the U4/U6.U5 tri-small nuclear ribonucleoprotein complex and several splicing factors that are commonly mutated in cancer. The interaction between cohesin and splicing factors/RBPs was RNA- and DNA-independent, occurred in chromatin, was enhanced during mitosis, and required RAD21. Furthermore, cohesin-interacting splicing factors and RBPs followed the cohesin cycle and prophase pathway of cell cycle-regulated interactions with chromatin. Depletion of cohesin-interacting splicing factors and RBPs resulted in aberrant mitotic progression. These results provide a comprehensive view of the endogenous human cohesin interactome and identify splicing factors and RBPs as functionally significant cohesin-interacting proteins.", "url": "https://www.ncbi.nlm.nih.gov/pubmed/31010829", "@type": ["Publication", "Item"], "ID": "PMID:31010829", "title": "Systematic proteomics of endogenous human cohesin reveals an interaction with diverse splicing factors and RNA-binding proteins required for mitotic progression.", "short_attribution": "Kim JS et al. (2019)", "journal": "The Journal of biological chemistry", "@id": "/publications/d5f08680-be3a-462b-a11a-73991e5b0ef1/", "display_title": "Kim JS et al. (2019) PMID:31010829", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "pubs_using": [], "publications_of_set": [{"uuid": "d5f08680-be3a-462b-a11a-73991e5b0ef1", "date_published": "2019-05-31", "journal": "The Journal of biological chemistry", "status": "current", "authors": ["Kim JS", "He X", "Liu J", "Duan Z", "Kim T", "Gerard J", "Kim B", "Pillai MM", "Lane WS", "Noble WS", "Budnik B", "Waldman T"], "@id": "/publications/d5f08680-be3a-462b-a11a-73991e5b0ef1/", "title": "Systematic proteomics of endogenous human cohesin reveals an interaction with diverse splicing factors and RNA-binding proteins required for mitotic progression.", "ID": "PMID:31010829", "display_title": "Kim JS et al. (2019) PMID:31010829", "@type": ["Publication", "Item"], "abstract": "The cohesin complex regulates sister chromatid cohesion, chromosome organization, gene expression, and DNA repair. Cohesin is a ring complex composed of four core  subunits and seven regulatory subunits. In an effort to comprehensively identify  additional cohesin-interacting proteins, we used gene editing to introduce a dual epitope tag into the endogenous allele of each of 11 known components of cohesin  in cultured human cells, and we performed MS analyses on dual-affinity purifications. In addition to reciprocally identifying all known components of cohesin, we found that cohesin interacts with a panoply of splicing factors and RNA-binding proteins (RBPs). These included diverse components of the U4/U6.U5 tri-small nuclear ribonucleoprotein complex and several splicing factors that are commonly mutated in cancer. The interaction between cohesin and splicing factors/RBPs was RNA- and DNA-independent, occurred in chromatin, was enhanced during mitosis, and required RAD21. Furthermore, cohesin-interacting splicing factors and RBPs followed the cohesin cycle and prophase pathway of cell cycle-regulated interactions with chromatin. Depletion of cohesin-interacting splicing factors and RBPs resulted in aberrant mitotic progression. These results provide a comprehensive view of the endogenous human cohesin interactome and identify splicing factors and RBPs as functionally significant cohesin-interacting proteins.", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "number_of_experiments": 2, "@context": "/terms/", "aggregated-items": {"badges": []}, "validation-errors": []}