ExperimentSetReplicate

ExperimentSet4DNES7UTO2B6
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Single molecule mrnaFISH on endogenous hunchback using dual color 5' and 3' block probe sets in heterozygotes of the hb deficiency

   November 5th, 2019 at 9:03am
Source Publication
Diverse Spatial Expression Patterns Emerge from Unified Kinetics of Transcriptional Bursting.
Zoller B, Little SC, et al., Cell 2018

Experiment Set Properties  

Set Type 
Replicate
Organism 
D. melanogaster
Sample Type 
whole organisms
Sample 
Fruit fly embryo hunchback +/- (NC13)
Experiment Type(s) 
RNA FISH
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: 8' region of hunchback mRNA (fruit fly), Chromosomes
Channel
Path
 
ch00
Chromosomes targeted by DAPI
ch01
5' region of hunchback mRNA (fruit fly) targeted by ATTO 565-labeled custom DNA oligos
ch02
3' region of hunchback mRNA (fruit fly) targeted by ATTO 633-labeled custom DNA oligos

7 Processed Files

Experiment
File
File Type
File Size
Experiment
4DNEXAWWFVUA
Bio Rep 1, Tec Rep 1
RNA FISH on Fruit fly embryo hunchback +/- (NC13)
File
4DNFI6A9UPLN
microscopy summary (csv)
43.18 kB
Experiment
4DNEX8VW4L4U
Bio Rep 2, Tec Rep 1
RNA FISH on Fruit fly embryo hunchback +/- (NC13)
File
4DNFI5BA8R9U
microscopy summary (csv)
49.19 kB
Experiment
4DNEX54SUJSO
Bio Rep 3, Tec Rep 1
RNA FISH on Fruit fly embryo hunchback +/- (NC13)
File
4DNFIE7II1IF
microscopy summary (csv)
38.38 kB
Experiment
4DNEXX34I2HV
Bio Rep 4, Tec Rep 1
RNA FISH on Fruit fly embryo hunchback +/- (NC13)
File
4DNFI6A6WCJR
microscopy summary (csv)
37.7 kB
Experiment
4DNEXLZ7VS3W
Bio Rep 5, Tec Rep 1
RNA FISH on Fruit fly embryo hunchback +/- (NC13)
File
4DNFIY4D8GBP
microscopy summary (csv)
46.7 kB
Experiment
4DNEXICCR87P
Bio Rep 6, Tec Rep 1
RNA FISH on Fruit fly embryo hunchback +/- (NC13)
File
4DNFICNLXR98
microscopy summary (csv)
45.63 kB
Experiment
4DNEXV1C817P
Bio Rep 7, Tec Rep 1
RNA FISH on Fruit fly embryo hunchback +/- (NC13)
File
4DNFI429BM58
microscopy summary (csv)
42.31 kB