ExperimentSetReplicate

ExperimentSet4DNESGCPT17E
released
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FISH/IF of Chromosome 4-Active and Chromosome 2-Active and Nuclear Speckle

   May 5th, 2018 at 4:05am
Source Publication
Higher-Order Inter-chromosomal Hubs Shape 3D Genome Organization in the Nucleus.
Quinodoz SA, Ollikainen N, et al., Cell 2018

Assay Description  

Experiment Set Properties  

Set Type 
Replicate
Organism 
M. musculus
Sample Type 
stem cells
Sample 
F1 2-1 mESC line
Experiment Type(s) 
DNA FISH
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: Chromosomes, GRCm38:4:149075743-149626036 mouse region, GRCm38:2:29844332-30444626 mouse region, Srsf2 mouse protein
Sample Image 
DNA FISH sample image
View File Item - 4DNFIX84K4WT
Channel
Path
 
ch00
Chromosomes targeted by DAPI
ch01
GRCm38:4:149075743-149626036 mouse region targeted by 495/517nm fluorophore-labeled oligo probe set
ch02
GRCm38:2:29844332-30444626 mouse region targeted by 547/565nm fluorophore-labeled oligo probe set
ch03
Srsf2 mouse protein targeted by mouse anti-SC35 antibody (with DyLight650-labeled anti-mouse secondary antibody)

1 Processed File

Experiment
File
File Type
File Size
Experiment
4DNEX76Z42QY
Bio Rep 1, Tec Rep 1
DNA FISH on F1 2-1 mESC line
File
4DNFISFEPZTV
locus distances, submitter format (csv)
2.1 kB