ExperimentSetReplicate

ExperimentSet4DNESIGBIXQS
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Chromosome tracing on 571 cells of a 210-kb genomic region containing Sox2 and super-enhancer (SE) loci in mouse F123 ESCs with an engineered replacement of 4 CTCF binding sites between Sox2 and SE on the CAST allele of the CAST X 129 hybrid background.

   October 14th, 2021 at 3:32pm
Source Publication
CTCF mediates dosage- and sequence-context-dependent transcriptional insulation by forming local chromatin domains.
Huang H, Zhu Q, et al., Nature genetics 2021

Experiment Set Properties  

Set Type 
Replicate
Organism 
M. musculus
Sample Type 
stem cells
Sample 
F123-CASTx129 with Sox2 tags and RMCE site between Sox2 and its SE
Experiment Type(s) 
multiplexed FISH
Modification Type 
RMCE
Treatment Type 
None
Assay Details 
Target: 42 consecutive 5-kb loci on chr3 (mouse), Chromosomes
Channel
Imaging Rounds
Path
 
ch04
0
Chromosomes targeted by DAPI
ch03
0-14
505/515nm fiducial beads
ch02
1-14
14 5-kb loci on chr3 (mouse) targeted by oligo probe library, readout probes (with Cy3-labeled imaging oligos).
ch01
1-14
14 5-kb loci on chr3 (mouse) targeted by oligo probe library, readout probes (with Cy5-labeled imaging oligos).
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1 Processed File

Experiment
File
File Type
File Size
Experiment
4DNEX63ENWXN
Bio Rep 1, Tec Rep 1
multiplexed FISH on F123-CASTx129 with Sox2 tags and RMCE site between Sox2 and its SE
File
4DNFI2RCYFJU
FOF-CT - DNA-spot/trace core (csv)
3.48 MB