ExperimentSetReplicate

ExperimentSet4DNESYARPKQ5
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Confocal microscopy experiments to compare the detection of TElomeric Repeat-containing RNA (TERRA) by RNA FISH and by dCas13-EGFP in U2OS cells

   November 4th, 2021 at 4:06pm
Source Publication
CRISPR Cas13-Based Tools to Track and Manipulate Endogenous Telomeric Repeat-Containing RNAs in Live Cells.
Xu M, Chigumira T, et al., Frontiers in molecular biosciences 2021

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
immortalized cells
Sample 
U2OS
Experiment Type(s) 
RNA FISH
Modification Type 
None
Treatment Type 
Transient Transfection
Assay Details 
Target: Chromosomes, TERRA
Channel
Path
 
ch00
Chromosomes targeted by DAPI
ch01
TERRA targeted by TERRA gRNA (with eGFP-labeled dCas13b)
ch02
TERRA targeted by Cy3-labeled custom DNA oligos

3 Processed Files

Experiment
File
File Type
File Size
Experiment
4DNEXUFSUI5Q
Bio Rep 1, Tec Rep 1
RNA FISH on U2OS
File
4DNFIGEDQHCX
tsv
801 Bytes
Experiment
4DNEXQY9UW31
Bio Rep 2, Tec Rep 1
RNA FISH on U2OS
File
4DNFIVAJ6HCB
tsv
648 Bytes
Experiment
4DNEXLI3QXR3
Bio Rep 3, Tec Rep 1
RNA FISH on U2OS
File
4DNFI9W3SDUW
tsv
475 Bytes