released
June 3rd, 2019 at 4:11pm
Reference Publication
Paulsen MT et al. (2014) PMID:23973811
Paulsen MT, Veloso A, et al., Methods (San Diego, Calif.) 2014
Overview
Experiment Category
Sequencing
Assay Classification
RNA Quantification
Experimental Purpose
Transcription
Raw Files Available
Reads (fastq) provided by lab
Assay Description
Bru-Seq is a technique to detect spans of nascent transcription in the genome.
Nascent RNA is labeled by a pulse of Bromouridine (BrU) and specifically captured
using anti-BrdU antibodies. cDNA libraries are produced from the isolated Bru-RNA and sequenced.
Sequences are aligned and analyzed to assess timing and level of transcription.