released
October 7th, 2019 at 3:49pm
Reference Publication
Li G et al. (2019) PMID:31384045
Li G, Liu Y, et al., Nature methods 2019
Overview
Experiment Category
Sequencing
Assay Classification
3C via Ligation
Experimental Purpose
DNA-DNA Pairwise Interactions
Raw Files Available
Reads (fastq) provided by lab
Assay Description
Methyl Hi-C is a method that combines in situ Hi-C and WGBS
to simultaneously profile chromatin conformation and DNA methylation.
It begins with a standard in situ Hi-C procedure, followed by an additional
step of bisulfite conversion before library construction and paired-end sequencing.
The methylation status of cytosines and the pair-wise contact frequencies are
then determined.