ExperimentHiC Dilution Hi-C on HeLa cell line with HindIII

Dilution Hi-C4DNEX3JYYRC6
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Hi-C: Scc1-AID WAB + auxin, 15 min

   February 20th, 2019 at 6:24pm
Source Publication
Topologically associating domains and chromatin loops depend on cohesin and are regulated by CTCF, WAPL, and PDS5 proteins.
Wutz G, Varnai C, et al., The EMBO journal 2017

Properties  

Experiment Type 
Dilution Hi-C
Follows SOP 
No
Biosample 
4DNBS5BA2WK1
Digestion Enzyme 
HindIII
Biosample Modifications 
Crispr generated mEGFP-tagged RAD21 and Crispr generated AID-tagged RAD21 and Tir1 random insertion
Biosample Treatments 
siRNA of WAPL gene and siRNA of PDS5A gene and siRNA of PDS5B gene and Auxin treatment (15.0m)
Biosample Biosource 
HeLa cell line - 4DNSRIZVADRC

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