ExperimentHiC in situ Hi-C on F123-CASTx129 (Tier 2) with MboI

in situ Hi-C4DNEX4J4Z9WE
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F123 CAST X 129 mouse cells containing a genomically modified AID-tagged CTCF treated with Auxin for 96 hours and then prepared for in situ Hi-C - Bioreplicate 2

   May 27th, 2018 at 4:58am
Source Publication
Promoter-proximal CTCF binding promotes distal enhancer-dependent gene activation.
Kubo N, Ishii H, et al., Nature structural & molecular biology 2021

Properties  

Experiment Type 
in situ Hi-C
Follows SOP 
No
Biosample 
4DNBSRIBBPGK
Digestion Enzyme 
MboI
Biosample Modifications 
Crispr generated AID-tagged CTCF and random integration of TIR1
Biosample Treatments 
Auxin treatment (0.5 mM, 96.0h)
Biosample Biosource 
F123-CASTx129 (Tier 2) - 4DNSRTNKUDSA

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