ExperimentHiC in situ Hi-C on K562 (Tier 2) with DpnII

in situ Hi-C4DNEXEK4WNHA
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Nuclei were purify from K562 cells, and then digest chromatin by adding 300U DpnII to 5M nuclei then incubated at 37C for 240 min before using Hi-C 2.0. After cross-linking I skip only the lysis step

   June 4th, 2019 at 2:10pm
Source Publication
Liquid chromatin Hi-C characterizes compartment-dependent chromatin interaction dynamics.
Belaghzal H, Borrman T, et al., Nature genetics 2021

Properties  

Experiment Type 
in situ Hi-C
Follows SOP 
No
Biosample 
4DNBSLXGZ3JR
Digestion Enzyme 
DpnII
Biosample Modifications 
None
Biosample Treatments 
None
Biosample Biosource 
K562 (Tier 2) - 4DNSRWQFKJYO

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