ExperimentMic DNA FISH on F1 2-1 mESC line

DNA FISH4DNEX1GI9T7B
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FISH/IF of Chromosome 19-Control and Chromosome 18-Control2 and Nucleolus and Nuclear Speckle

   May 5th, 2018 at 4:04am
Source Publication
Higher-Order Inter-chromosomal Hubs Shape 3D Genome Organization in the Nucleus.
Quinodoz SA, Ollikainen N, et al., Cell 2018

Properties  

Experiment Type 
DNA FISH
Follows SOP 
No
Biosample 
4DNBS89JOZSF
Biosample Biosource 
F1 2-1 mESC line - 4DNSRW5UG3MR
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Path
 
ch00
GRCm38:19:44925701-46925611 mouse region targeted by 429/470nm fluorophore-labeled oligo probe set
440nm
ch01
GRCm38:18:75840470-76761410 mouse region targeted by 495/517nm fluorophore-labeled oligo probe set
470nm
ch02
Ncl mouse protein targeted by rabbit anti-nucleolin antibody (with Alexa Fluor 568-labeled anti-rabbit secondary antibody)
550nm
ch03
Srsf2 mouse protein targeted by mouse anti-SC35 antibody (with DyLight650-labeled anti-mouse secondary antibody)
640nm

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