ExperimentMic RNA FISH on G1E-ER4 with random integration of ER-responsive GATA-1, GATA-1 gene deletion, Crispr deletion of Slc25a37 enhancer E1

RNA FISH4DNEX2W7MCLI
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RNA FISH of Slc25a37 intronic and exonic regions in e1-deleted G1-ER4 cells from Jian Xu lab differentiated for 24 hours

   September 14th, 2018 at 4:50pm
Source Publication
Transcriptional Burst Initiation and Polymerase Pause Release Are Key Control Points of Transcriptional Regulation.
Bartman CR, Hamagami N, et al., Molecular cell 2018

Properties  

Experiment Type 
RNA FISH
Follows SOP 
No
Biosample 
4DNBSM4V5WN5
Biosample Biosource 
G1E-ER4 with random integration of ER-responsive GATA-1, GATA-1 gene deletion, Crispr deletion of Slc25a37 enhancer E1 - 4DNSR3QZPFQ9
Biosample Modifications 
None
Biosample Treatments 
estradiol treatment (100 nM, 24h)
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Path
 
ch00
Chromosomes targeted by DAPI
ch01
GRCm38:14:69241851-69285103 mouse region targeted by Cy5-labeled custom oligos
ch02
GRCm38:14:69241851-69285103 mouse region targeted by Cy3-labeled custom oligos

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