ExperimentMic RNA FISH on G1E-ER4 with random integration of ER-responsive GATA-1, GATA-1 gene deletion

RNA FISH4DNEX5RGMJD2
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RNA FISH of Zfpm1 and Pabpc1 intronic regions in G1E-ER4 cells differentiated for 24 hours

   September 14th, 2018 at 4:52pm
Source Publication
Transcriptional Burst Initiation and Polymerase Pause Release Are Key Control Points of Transcriptional Regulation.
Bartman CR, Hamagami N, et al., Molecular cell 2018

Properties  

Experiment Type 
RNA FISH
Follows SOP 
No
Biosample 
4DNBSEDU5S31
Biosample Biosource 
G1E-ER4 with random integration of ER-responsive GATA-1, GATA-1 gene deletion - 4DNSR41SLMH6
Biosample Modifications 
None
Biosample Treatments 
estradiol treatment (100 nM, 24h)
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Path
 
ch00
Chromosomes targeted by DAPI
ch01
GRCm38:8:122282141-122337247 mouse region targeted by Alexa Fluor 594-labeled custom oligos
ch02
GRCm38:15:36595658-36608973 mouse region targeted by Cy3-labeled custom oligos

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