ExperimentMic multiplexed FISH on F123-CASTx129 with Sox2 tags and RMCE site between Sox2 and its SE

multiplexed FISH4DNEX63ENWXN
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Imaging the 7.5kb insert to visualize chromatin structural changes in the presence of the synthetic insulator at the Sox2 locus known to reduce transcription of the Sox2 gene.

   October 14th, 2021 at 3:32pm
Source Publication
CTCF mediates dosage- and sequence-context-dependent transcriptional insulation by forming local chromatin domains.
Huang H, Zhu Q, et al., Nature genetics 2021

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSCHNIPZS
Biosample Biosource 
F123-CASTx129 with Sox2 tags and RMCE site between Sox2 and its SE - 4DNSR9TIV5EV
Biosample Modifications 
RMCE replacement
Biosample Treatments 
None
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Imaging Rounds
Path
 
ch04
0
Chromosomes targeted by DAPI
ch03
0-14
505/515nm fiducial beads
ch02
1-14
14 5-kb loci on chr3 (mouse) targeted by oligo probe library, readout probes (with Cy3-labeled imaging oligos).
ch01
1-14
14 5-kb loci on chr3 (mouse) targeted by oligo probe library, readout probes (with Cy5-labeled imaging oligos).
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