ExperimentMic RNA FISH on U2OS

RNA FISH4DNEX6BLN69C
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Confocal microscopy experiment for TERRA FISH to compare detected TERRA by dCas13-Suntag-sfGFP in U2OS cells, replicate 3

   November 4th, 2021 at 4:06pm
Source Publication
CRISPR Cas13-Based Tools to Track and Manipulate Endogenous Telomeric Repeat-Containing RNAs in Live Cells.
Xu M, Chigumira T, et al., Frontiers in molecular biosciences 2021

Properties  

Experiment Type 
RNA FISH
Follows SOP 
No
Biosample 
4DNBSYZ2WXVP
Biosample Biosource 
U2OS - 4DNSRXN5YHHZ
Biosample Modifications 
None
Biosample Treatments 
Transient transfection of dCas13-Suntag-sfGFP, TERRA-gRNA
Microscopy Technique 
Spinning-Disk Confocal
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Path
 
ch00
Chromosomes targeted by DAPI
ch01
TERRA targeted by TERRA gRNA, dCas13b-SunTag (with sfGFP-labeled scFv)
ch02
TERRA targeted by Cy3-labeled custom DNA oligos

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