ExperimentMic DNA FISH on F1 2-1 mESC line

DNA FISH4DNEXD5YH1G8
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FISH/IF of Chromosome 12-NOR and Chromosome 19-NOR and Nucleolus

   May 5th, 2018 at 4:03am
Source Publication
Higher-Order Inter-chromosomal Hubs Shape 3D Genome Organization in the Nucleus.
Quinodoz SA, Ollikainen N, et al., Cell 2018

Properties  

Experiment Type 
DNA FISH
Follows SOP 
No
Biosample 
4DNBS9DTH16U
Biosample Biosource 
F1 2-1 mESC line - 4DNSRW5UG3MR
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Path
 
ch00
Chromosomes targeted by DAPI
395nm
ch01
GRCm38:12:5993048-6993343 mouse region targeted by 495/517nm fluorophore-labeled oligo probe set
470nm
ch02
GRCm38:19:17928145-19921671 mouse region targeted by 547/565nm fluorophore-labeled oligo probe set
550nm
ch03
Ncl mouse protein targeted by rabbit anti-nucleolin antibody (with Alexa Fluor 647-labeled anti-rabbit secondary antibody)
640nm

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