ExperimentMic multiplexed FISH on liver

multiplexed FISH4DNEXP7EQ66C
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Multiplexed Imaging of Nucleome Architectures in fetal mouse liver, including chromatin tracing of Chr19, RNA MERFISH, and mapping nucleolar and lamina associations

   March 15th, 2021 at 11:39pm
Source Publication
Multiplexed imaging of nucleome architectures in single cells of mammalian tissue.
Liu M, Lu Y, et al., Nature communications 2020

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBS67CMNGR
Biosample Biosource 
liver - 4DNSRA2P2JSA
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
None
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Imaging Rounds
Path
 
ch00
41
Chromosomes targeted by DAPI
ch01
0-40
505/515nm fiducial beads
ch02
1-19
19 consecutive 5-kb loci on chr19 (mouse) targeted by oligo probe library (with ATTO 565-labeled imaging oligos)
ch02
20
Cell membrane targeted by oligo-conjugated WGA (with ATTO 565-labeled imaging oligo)
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