ExperimentMic multiplexed FISH on F123-CASTx129 with Sox2 tags and RMCE site between Sox2 and its SE

Imaging the 7.5kb insert to visualize chromatin structural changes in the presence of the synthetic insulator at the Sox2 locus known to reduce transcription of the Sox2 gene. We also colocalized the chromatin data with nascent RNA images to understand bursting behavior as a function of the allele classification.