ExperimentMic multiplexed FISH on F123-CASTx129 with Sox2 tags and RMCE site between Sox2 and its SE

multiplexed FISH4DNEXZZJG24L
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Imaging a blank (no CTCF binding sites) 7.5kb insert as a control to confirm that the chromatin structural changes are not a result of the insert itself, but caused by the CTCF binding sites and flanking regions themselves.

   October 14th, 2021 at 3:32pm
Source Publication
CTCF mediates dosage- and sequence-context-dependent transcriptional insulation by forming local chromatin domains.
Huang H, Zhu Q, et al., Nature genetics 2021

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSZTCUG9B
Biosample Biosource 
F123-CASTx129 with Sox2 tags and RMCE site between Sox2 and its SE - 4DNSR9TIV5EV
Biosample Modifications 
RMCE replacement
Biosample Treatments 
None
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Imaging Rounds
Path
 
ch04
0
Chromosomes targeted by DAPI
ch03
0-14
505/515nm fiducial beads
ch02
1-14
14 5-kb loci on chr3 (mouse) targeted by oligo probe library, readout probes (with Cy3-labeled imaging oligos).
ch01
1-14
14 5-kb loci on chr3 (mouse) targeted by oligo probe library, readout probes (with Cy5-labeled imaging oligos).
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