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To investigate the impact of transcribed sequence on elongation potential, we developed a method to screen the effects of thousands of INtegrated Sequences on Expression of RNA and Translation using high-throughput sequencing (INSERT-seq). We found that higher AT content in uaRNAs and eRNAs, rather than specific sequence motifs, underlies the propensity for RNAPII termination on these transcripts. Further, we demonstrate that 5 splice sites exert both splicing-dependent and autonomous, splicing-independent stimulation of transcription, even in the absence of polyadenylation signals. Together, our results reveal a potent role for transcribed sequence in dictating gene output at mRNA and non-coding RNA loci, and demonstrate the power of INSERT-seq towards illuminating these contributions.", "date_published": "2021-06-01", "title": "Screening thousands of transcribed coding and non-coding regions reveals sequence determinants of RNA polymerase II elongation potential", "ID": "doi:10.1101/2021.06.01.446655", "@type": ["Publication", "Item"], "journal": "bioRxiv", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "publications_of_exp": [{"uuid": "5d4f2c86-bd45-422f-b787-f04f2c9c12c6", "@id": "/publications/5d4f2c86-bd45-422f-b787-f04f2c9c12c6/", "authors": ["Vlaming H", "Mimoso CA", "Martin BJ", "Field AR", "Adelman K"], "title": "Screening thousands of transcribed coding and non-coding regions reveals sequence determinants of RNA polymerase II elongation potential", "ID": "doi:10.1101/2021.06.01.446655", "@type": ["Publication", "Item"], "display_title": "Vlaming H et al. 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To investigate the impact of transcribed sequence on elongation potential, we developed a method to screen the effects of thousands of INtegrated Sequences on Expression of RNA and Translation using high-throughput sequencing (INSERT-seq). We found that higher AT content in uaRNAs and eRNAs, rather than specific sequence motifs, underlies the propensity for RNAPII termination on these transcripts. Further, we demonstrate that 5 splice sites exert both splicing-dependent and autonomous, splicing-independent stimulation of transcription, even in the absence of polyadenylation signals. 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