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TSA-seq comparing speckle/active regions and lamin-associated regions

The top 2 tracks show TSA-seq using independent markers of nuclear speckles: SON and phosphorylated SC35. The middle track uses an antibody that detects a poised version of RNA Pol II subunit as a marker of actively transcribed regions. While the bottom 2 tracks are for Lamins markers of the nuclear lamina. The display below highlights the finding that actively transcribing regions and nuclear speckles tend to have similar TSA-seq profiles that are generally inverse to profiles using markers for the more inactive lamina regions.