Modification

Crispr deletion of SCC4

CRISPRs targeting SCC4 (5’-CACCGTACGGGCCTCGATGCGCTG-3’) were cloned into px330 (Addgene plasmid #42230). ∆SCC4 HAP1 clones were generated by insertion of a Puromycin cassette as previously described (Blomen et al., 2015).