released
May 11th, 2017 at 3:50pm
Details
Details
definition
The DT40 chicken B cell line is derived from an avian leukosis virus (ALV)-induced bursal lymphoma. The ALV was injected intravenously into Hyline SC chickens and a resulting tumor was twice transplanted in vivo prior to cell culturing (Baba and Humphries, 1984; Baba et al., 1985). The DT40 cells are small, approximately 10 μm in diameter, and have a high nucleus to cytoplasm ratio (Fig. 1). They carry IgM on the surface, and although Baba et al. (1985) did not find evidence of secreted IgM, others have (Takagaki et al., 1996; Takami et al., 1999). DT40 cells do release ALV into the surroundings, but in negligible amounts (Baba et al., 1985). In keeping with the observation that nearly 85% of all ALV-induced tumors are disrupted in the c-myc locus, the DT40 cells have integrated the 3′ viral long terminal repeat (LTR) upstream of the c-myc gene ( Hayward et al., 1981). The 3′ LTR is in the same orientation as c-myc, elevating the transcription levels 100-fold in this cell line compared with normal avian bursal cells ( Baba et al., 1985). Whereas Baba et al. (1985) detected a normal and an altered c-myc allele, Kim et al. (1990) reported a possible mitotic recombination event among the parental alleles, as they describe the loss of the normal c-myc allele and instead find two copies of the altered c-myc gene. DT40 cells continue to undergo gene conversion, as seen by testing for the presence or absence of various restriction sites within the light chain variable region ( Thompson et al., 1987). Although the DT40 cell line may best be characterized as being a bursal B cell line, the ongoing gene conversion and the fact that v-myc transformed cells can reconstitute an ablated bursa of Fabricius suggests, that the DT40 cell line is arrested at a bursal stem cell stage of differentiation ( Baba et al., 1985; Thompson et al., 1987; McCormack et al., 1991). (EFO)
namespace
http://www.ebi.ac.uk/efo
parents
preferred_name
DT40
references
- None
source_ontologies
term_id
EFO:0006274
term_name
DT40
term_url
http://www.ebi.ac.uk/efo/EFO_0006274