Joint profiling of DNA methylation and chromatin architecture in single cells.

Abstract

We report a molecular assay, Methyl-HiC, that can simultaneously capture the chromosome conformation and DNA methylome in a cell. Methyl-HiC reveals coordinated DNA methylation status between distal genomic segments that are in spatial proximity in the nucleus, and delineates heterogeneity of both the chromatin architecture and DNA methylome in a mixed population. It enables simultaneous characterization of cell-type-specific chromatin organization and epigenome in complex tissues.

Authors

Li G  •  Liu Y  •  Zhang Y  •  Kubo N  •  Yu M  •  Fang R  •  Kellis M  •  Ren B

Link

https://www.ncbi.nlm.nih.gov/pubmed/31384045