Trac-looping measures genome structure and chromatin accessibility.

Abstract

Long-range chromatin interactions play critical roles in genome organization and regulation of transcription. We now report transposase-mediated analysis of chromatin looping (Trac-looping) for simultaneous detection of multiscale genome-wide chromatin interactions among regulatory elements and chromatin accessibility. With this technique, a bivalent oligonucleotide linker is inserted between two interacting regions such that the chromatin interactions are captured without prior chromatin fragmentation and proximity-based ligation. Application of Trac-looping to human CD4(+) T cells revealed substantial reorganization of enhancer-promoter interactions associated with changes in gene expression after T cell receptor stimulation.

Authors

Lai B  •  Tang Q  •  Jin W  •  Hu G  •  Wangsa D  •  Cui K  •  Stanton BZ  •  Ren G  •  Ding Y  •  Zhao M  •  Liu S  •  Song J  •  Ried T  •  Zhao K

Link

https://www.ncbi.nlm.nih.gov/pubmed/30150754