{"ID": "PMID:31010829", "aka": "https://www.biorxiv.org/content/early/2018/05/17/325209", "lab": {"@type": ["Lab", "Item"], "display_title": "Todd Waldman, GEORGETOWN", "uuid": "7aee8214-d09b-4d55-98da-66fa020ce05a", "status": "current", "title": "Todd Waldman, GEORGETOWN", "@id": "/labs/todd-waldman-lab/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "submits_for.7aee8214-d09b-4d55-98da-66fa020ce05a"]}}, "url": "https://www.ncbi.nlm.nih.gov/pubmed/31010829", "award": {"@id": "/awards/external-award/", "display_title": "EXTERNAL AWARD", "status": "current", "center_title": "External", "@type": ["Award", "Item"], "center": "External", "name": "external-award", "project": "External", "description": "Funding source is from outside 4DN.", "uuid": "12a92962-8265-4fc0-b2f8-cf14f05db58b", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "title": "Systematic proteomics of endogenous human cohesin reveals an interaction with diverse splicing factors and RNA-binding proteins required for mitotic progression.", "status": "current", "authors": ["Kim JS", "He X", "Liu J", "Duan Z", "Kim T", "Gerard J", "Kim B", "Pillai MM", "Lane WS", "Noble WS", "Budnik B", "Waldman T"], "journal": "The Journal of biological chemistry", "abstract": "The cohesin complex regulates sister chromatid cohesion, chromosome organization, gene expression, and DNA repair. Cohesin is a ring complex composed of four core  subunits and seven regulatory subunits. In an effort to comprehensively identify  additional cohesin-interacting proteins, we used gene editing to introduce a dual epitope tag into the endogenous allele of each of 11 known components of cohesin  in cultured human cells, and we performed MS analyses on dual-affinity purifications. In addition to reciprocally identifying all known components of cohesin, we found that cohesin interacts with a panoply of splicing factors and RNA-binding proteins (RBPs). These included diverse components of the U4/U6.U5 tri-small nuclear ribonucleoprotein complex and several splicing factors that are commonly mutated in cancer. The interaction between cohesin and splicing factors/RBPs was RNA- and DNA-independent, occurred in chromatin, was enhanced during mitosis, and required RAD21. Furthermore, cohesin-interacting splicing factors and RBPs followed the cohesin cycle and prophase pathway of cell cycle-regulated interactions with chromatin. Depletion of cohesin-interacting splicing factors and RBPs resulted in aberrant mitotic progression. These results provide a comprehensive view of the endogenous human cohesin interactome and identify splicing factors and RBPs as functionally significant cohesin-interacting proteins.", "categories": ["basic biology"], "date_created": "2019-06-28T13:29:44.045780+00:00", "published_by": "External", "submitted_by": {"error": "no view permissions"}, "last_modified": {"modified_by": {"error": "no view permissions"}, "date_modified": "2019-06-28T13:29:44.942596+00:00"}, "date_published": "2019-05-31", "public_release": "2019-06-28", "schema_version": "2", "project_release": "2019-06-28", "exp_sets_prod_in_pub": [{"@id": "/experiment-set-replicates/4DNES5OZGN2L/", "experimentset_type": "replicate", "@type": ["ExperimentSetReplicate", "ExperimentSet", "Item"], "uuid": "50735160-b306-4671-8966-4f78f567b08b", "status": "released", "display_title": "4DNES5OZGN2L", "accession": "4DNES5OZGN2L", "experiments_in_set": [{"@type": ["ExperimentHiC", "Experiment", "Item"], "display_title": "DNase Hi-C on HeLa-S3 with DNaseI - 4DNEXBXZVFAN", "@id": "/experiments-hi-c/4DNEXBXZVFAN/", "status": "released", "uuid": "8c917004-2425-4184-8ed0-e763b1cee119", "experiment_type": {"title": "DNase Hi-C", "@type": ["ExperimentType", "Item"], "status": "released", "@id": "/experiment-types/dnase-hi-c/", "uuid": "eb3f2a22-fa89-497a-842c-3317347c5f88", "display_title": "DNase Hi-C", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, {"@type": ["ExperimentHiC", "Experiment", "Item"], "display_title": "DNase Hi-C on HeLa-S3 with DNaseI - 4DNEX7TC67QU", "@id": "/experiments-hi-c/4DNEX7TC67QU/", "status": "released", "uuid": "e8535cdc-1033-4c36-9b96-299d32766717", "experiment_type": {"title": "DNase Hi-C", "@type": ["ExperimentType", "Item"], "status": "released", "@id": "/experiment-types/dnase-hi-c/", "uuid": "eb3f2a22-fa89-497a-842c-3317347c5f88", "display_title": "DNase Hi-C", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, {"@id": "/experiment-set-replicates/4DNESGEEV6TJ/", "experimentset_type": "replicate", "@type": ["ExperimentSetReplicate", "ExperimentSet", "Item"], "uuid": "4dc71f70-91b5-4cf1-8322-262be13d877d", "status": "released", "display_title": "4DNESGEEV6TJ", "accession": "4DNESGEEV6TJ", "experiments_in_set": [{"@type": ["ExperimentHiC", "Experiment", "Item"], "display_title": "DNase Hi-C on HeLa-S3 with DNaseI - 4DNEXSRILLQI", "@id": "/experiments-hi-c/4DNEXSRILLQI/", "status": "released", "uuid": "6744c3f9-fb7e-489f-894a-ac705ba9f847", "experiment_type": {"title": "DNase Hi-C", "@type": ["ExperimentType", "Item"], "status": "released", "@id": "/experiment-types/dnase-hi-c/", "uuid": "eb3f2a22-fa89-497a-842c-3317347c5f88", "display_title": "DNase Hi-C", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, {"@type": ["ExperimentHiC", "Experiment", "Item"], "display_title": "DNase Hi-C on HeLa-S3 with DNaseI - 4DNEXZ3C2HEZ", "@id": "/experiments-hi-c/4DNEXZ3C2HEZ/", "status": "released", "uuid": "b128f8c7-f15f-449e-a1cf-934da62cd08e", "experiment_type": {"title": "DNase Hi-C", "@type": ["ExperimentType", "Item"], "status": "released", "@id": "/experiment-types/dnase-hi-c/", "uuid": "eb3f2a22-fa89-497a-842c-3317347c5f88", "display_title": "DNase Hi-C", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "@id": "/publications/d5f08680-be3a-462b-a11a-73991e5b0ef1/", "@type": ["Publication", "Item"], "uuid": "d5f08680-be3a-462b-a11a-73991e5b0ef1", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "display_title": "Kim JS et al. 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